Optimizing Cell Migration Scratch Assays With Effective Post-Scratch Microplate Washing

The scratch assay consists of removing a region of cells from a monolayer and then monitoring the movement of neighboring cells into the cleared area. After wounds are formed, it's critical to remove cellular debris generated during this process to prevent it from settling back down into the wound area.

BioTek AutoScratch wound making tool automatically creates reproducible uniform scratch wounds for cell migration and invasion studies. Automated, customizable protocols in BioTek MultiFlo FX multimode dispenser enable it to quickly wash microplates removing cellular debris for fast, effective results.