Using the Epoch & Take3 Plate

This product demo describes the use of BioTek's Take3 plate and the Epoch microplate spectrophotometer to simultaneously read sixteen 2 µL samples.

Script:

Today’s laboratories are searching for ways to use lower reagent and sample volumes, while increasing throughput and efficiency to keep costs in check.

We’ll demonstrate the use of BioTek’s Take3 Multi-Volume plate with the Epoch Microplate spectrophotometer to quantify multiple DNA samples simultaneously, using only 2 µL of sample.

BioTek’s Take3 plate has a simple but innovative design for measuring multiple low volume samples, which is particularly useful for direct nucleic acid and protein quantification.

The Take3 plate has two uniquely designed fused silica slides mounted by magnets to the housing. The base slide is printed with Teflon to form sixteen 2 mm diameter ‘microspots’. The microspots are the ideal size for pipetting samples as small as 2 microliters. When the lid of the Take3 is closed, the samples are sandwiched between top and bottom slides, held to a nominal 0.5 mm pathlength for each microspot.

The absorbance of the samples in the Take3 plate can be measured using any BioTek microplate spectrophotometer - the fixed pathlengths of the plate allow rapid calculation of concentrations based on a standard 1 cm pathlength.

We’re going to use the Take3 plate with the Epoch reader, a compact plate reader with 200 - 999 nm wavelength range.

Pre-programmed protocols in the Gen5 software make it simple to choose the sample type and quickly obtain and calculate data from the Take3 plate.

We’ll use an 8 channel pipettor to place our blanks and DNA samples on the Take3 plate.

Open a Take3 session in Gen5 and select the sample type, either single or double-stranded DNA. We’re using double stranded DNA samples, each with their own buffer blank so all microspots will contain blanks.

After the blanks are read, the Take3 plate is removed and the blanks are wiped off both top and bottom slides with a lab wipe.

Next, pipette the samples onto the microspots. Select all sixteen locations and read the plate. The results are calculated automatically and displayed in an Excel sheet. Raw and calculated data are given as is the blank well information.

You can see that using Take3 makes quantification of one or more 2 µL samples fast, easy and accurate.

The Take3 plate and Epoch reader used as a system provides great flexible to support all your laboratory needs - from low volume nucleic acid quantification to biomolecular assays in 96 well plates.

 

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