Resources - Webinars

LAMP Assays: Kinetic characterization using a Multimode Microplate Reader - On Demand

Related Products: Synergy H1, Synergy Neo2

August 03, 2022

Presenter: Peter Brescia, Applications Specialist

Isothermal nucleic acid amplification tests, such as Loop-mediated isothermal amplification (LAMP) have emerged over the past 20 years as an alternative to polymerase chain reaction (PCR). The use of LAMP assay technology spans a range of applications including its use as an important diagnostic tool in clinical applications, and food quality control and environmental monitoring. LAMP has gained traction for its ability to detect nucleic acid sequences without specialized equipment using a variety of detection modes. LAMP assays are typically run as an end point reaction in PCR tubes. In this larger format, observation for positive/negative determinations can be made manually via visualization of a colorimetric change due to pH. This methodology lends itself to adaptation for automated kinetic monitoring in a higher-density microplate format at low volumes.

In this webinar we will present:

  • Background and benefits of LAMP compared to conventional PCR
  • Guidelines and tips for adapting LAMP assays to a kinetic microplate-based format
  • Microplate reader settings for LAMP assay optimization