Resources - Webinars

High Throughput Imaging of 3D Engineered Model Constructs - On Demand

Related Products: Cytation 5

October 28, 2022

Presenters: Jordan Miner, Ph.D., Student in Biomedical Engineering; University of Maine Graduate School of Biomedical Sciences and Engineering and Karissa Tilbury, Ph.D., Assistant Professor; University of Maine Graduate School of Biomedical Sciences and Engineering

The native extracellular matrix (ECM) is a dynamic scaffold that maintains homeostasis through a complex milieu of biochemical and biomechanical interactions. 3D engineering tissue models provide the ability to tune parameters to identify and model the complex relationships between cellular and non-cellular components involved in development, wound healing, aging, and tumor initiation and promotion. The creation of 3D cancer models is an active field and is particularly relevant in revealing the impact of collagen remodeling and cellular metabolism of both cancerous, stromal, and infiltrating immune cells. The evolution of efficient high-throughput assays with spatial and temporal resolution provides the basis of a bi-directional feedback loop critical for identifying and screening new therapeutic targets.

In this webinar, the high-throughput, rapid screening methodologies developed for these 3D models are discussed for three separate projects demonstrating the vast capabilities of the Agilent BioTek Cytation 5 cell imaging multimode reader. These projects are:

  • Developing a collagen degradation model to explore the relationship between collagen remodeling in the ECM and a collagen cryptic α10 integrin binding site shown to modulate cellular behavior.
  • Exploring weak auto-fluorescent signals of NAD(P)H and FAD to probe the metabolic and proliferation states of breast cancer spheroids.
  • Characterizing cellulose nanofibers (CNF) as a potential biomaterial for bone regrowth applications.