Resources - Webinars

Biosensors for Cell Signaling: Tips and Tricks on how to obtain and analyze high quality data from live cell, high throughput assays of signaling dynamics - On Demand

Related Products: 405 TS, Cytation C10, Synergy Neo2

September 02, 2022

Presenters: Luciana Leo, Ph.D. - Montana Molecular, Bozeman, MT; Sam Hoare, Ph.D. - Montana Molecular, Bozeman, MT & Pharmechanics LLC, Owego, NY

Genetically encoded fluorescent biosensors enable continuous recording of cellular signaling in live cells. This breakthrough technology provides unprecedented, real-time measurements of   signaling dynamics, which is emerging as a critical determinant of physiological function and therapeutic targeting of signaling proteins. In this webinar we will go through the scientific and technological tips and tricks to obtain high quality, high throughput kinetic data on BioTek plate readers, with biosensors for G-protein-coupled receptor second messengers and arrestin recruitment. We will then describe how to analyze signaling dynamics by applying curve fitting to the time course data. This analysis is straightforward, and we provide a plug-in for the curve-fitting program GraphPad Prism to enable analysis with familiar software. This reveals new insights exemplified by differential signaling kinetics and arrestin recruitment mechanisms for multiple GPCRs.

Key Learning Objectives:

  • Technological tips and tricks to obtain high quality, high throughput kinetic data on BioTek plate readers
  • How to analyze signaling dynamics by applying curve fitting to the time course data

link