Whole-Mount Immunofluorescence Imaging of Zebrafish Eye Structures

Fluorescence immunohistochemistry is a primary technique used in zebrafish imaging research workflows. Optical sectioning is essential for high-quality imaging of deep tissue structures in whole-mount zebrafish, such as the retina and lens of the eye. Spinning-disk confocal microscopy overcomes imaging limitations of widefield microscopy for zebrafish whole-mount immunofluorescence.