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Viral Plaque Assay

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August 19, 2020

The viral plaque assay remains one of the most commonly used procedures to quantify infectious virions and antiviral neutralization strategies. The target cells are grown to full confluence on a microplate and infected with serial dilutions of virus. The viral infection causes the initial cells and surrounding cells to lyse. An agarose overlay prevents the infection from spreading outside of the localized clearing, or plaque. Following infection, the monolayer is fixed and stained with crystal violet to allow quantification of individual plaques in each well using automated image capture and analysis.

Viral Plaque Assay