Resources - Visual Abstracts

Screening Assay for Assessing Attachment Phenotype and Relative Total Biomass in Bacterial Biofilms

Related Products: Cytation 5, Cytation 7, Cytation C10, Epoch 2, Epoch, Synergy HTX, Synergy Neo2

October 28, 2022

A biofilm life cycle begins when bacterial cells adapt from a planktonic state to being irreversibly attached to a surface. A common screening method to assess this uses crystal violet staining. This technique results in both a qualitative visualization of cell attachment on a substrate and a relative quantitative measure of total attached biomass. The principle is demonstrated here on two dilutions of four bacterial strains assayed at multiple incubation time points.