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Resources - Visual Abstracts

Gram Stain Imaging

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March 19, 2018

Gram staining classifies bacterial strains based on differences in their cell wall. Green fluorescence results when CF™ 488A-Wheat Germ Agglutinin (WGA) binds to N-Acetyl Glucosamine in bacterial peptidoglycan. Gram positive bacteria appear bright green as they have a thick, exposed peptidoglycan layer. An outer membrane and thin peptidoglycan layer restrict the signal in gram negative bacteria.