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  Use our Product Finder to view and compare products that meet your specific requirements.
- Imaging & Microscopy
  Automated Cell Imagers
  
  Lionheart FX Automated Microscope
  Lionheart LX Automated Microscope
  Cell Imaging Multi-Mode Readers
  
  Cytation 5 Cell Imaging Multi-Mode Reader
  Cytation 1 Cell Imaging Multi-Mode Reader
  Multi-Plate Live Cell Imaging System
  
  BioSpa Live Cell Analysis System
  Peripherals
  
  Scratch Assay Starter Kit
  Gas Controller
  Dual Reagent Injector Module
  Product
  Comparison Charts
  
  Imaging & Microscopy
  
  Gen5 Edition Comparison
- Detection
  Multi-Mode Microplate Readers
  
  Synergy Neo2 Hybrid Multi-Mode Reader
  Cytation Hybrid Multi-Mode Reader
  Synergy H1 Hybrid Multi-Mode Reader
  Synergy HTX Multi-Mode Reader
  Synergy LX Multi-Mode Reader
  Absorbance Microplate Readers
  
  Epoch 2 Microplate Spectrophotometer
  Epoch Microplate Spectrophotometer
  PowerWave HT Microplate Spectrophotometer
  ELx808 Absorbance Reader
  800 TS Absorbance Reader
  Peripherals
  
  Gas Controller
  Take3 Plate
  Dual Reagent Injector Module
  Product Comparison Charts
  
  Multi-Mode Readers
  
  Absorbance Readers
  
  Microplate Spectrophotometers
  
  Gen5 Edition Comparison
- Liquid Handling
  Multi-Mode Washer Dispensers
  
  EL406 Washer Dispenser
  MultiFlo FX Multi-Mode Dispenser
  Microplate Washers
  
  405 TS Washer
  405 LS Washer
  ELx405 Select Deep Well Washer
  50 TS Washer
  Microplate Dispensers
  
  MultiFlo FX Multi-Mode Dispenser
  MicroFill Dispenser
  Product Comparison Charts
  
  Microplate Washers
  
  Microplate Dispensers
- Software
  Software
  
  Gen5 Software Features for Imaging & Microscopy
  Gen5 Software Features for Detection
  Liquid Handling Control Software
  Software Apps
  
  Scratch Assay App
  Quantitative Assay Apps
  Product Comparison Charts
  
  Gen5 Edition Comparison
- Robotics
  Robotics
  
  BioSpa 8 Automated Incubator
  BioStack Microplate Stacker
  Product Comparison Charts
  
  BioStack Configuration Comparison
- Technologies
  Multi-Mode Detection Technologies
  
  Absorbance
  Fluorescence Intensity
  Fluorescence Polarization
  Luminescence
  Time Resolved Fluorescence
  Imager & Microplate Reader Technologies
  
  4-Zone Incubation and Condensation Control
  Augmented Microscopy
  Hybrid Technology
  Laser Autofocus
  Peltier Cooling Module
  Variable Bandwidth Monochromators
  Liquid Handling Technologies
  
  Automated, Gentle Media Exchange
  Dual-Action Manifold
  Parallel Dispense
  Random Access Dispense
  Ultrasonic Advantage
  Verify Technology
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Peripherals
- Peripherals
  BioTek’s peripheral products help to expand your BioTek instrument applications and improve workflow efficiency. From gas controllers to micro-volume plates, we have the peripherals to help advance your research.
- Accessories
  To complete or enhance your BioTek imager, microplate reader, liquid handling or robotics system, we offer a wide range of accessories, from tubing and bottles to imaging filter cubes. Search our Accessory database for Accessories compatible with your instrument. Search by Keyword, Part #, Product or Accessory Type.
- Application Starter Kits
  BioTek’s Application Starter kits contain everything you’ll need to implement the latest assays for a particular application, quickly and with minimal training. Kits contain hardware, software, consumables and reagents all in a convenient kit.
Applications
- Imaging & Microscopy
  Imaging & Microscopy
  
  3D Cell Culture
  Apoptosis
  Cell Counting
  Cell Counting: Label-Free
  Cell Migration/Invasion
  Cell Metabolism
  Cell Proliferation
  Cell Viability/Toxicity
  Confluence
  Fast Kinetics
  Genotoxicity
  Histology
  Immunofluorescence
  Live Cell Imaging
  Microbiology
  Phenotypic Assays
  Stem Cell Differentiation
  Transfection Efficiency
  Whole Organism Imaging
- Detection & Liquid Handling
  Detection & Liquid Handling
  
  Biomarker Assays
  Cell-based Assays
  Cellular and Microbial Growth
  Cell Metabolism
  ELISA and Related Immunoassays
  Nucleic Acid Quantification
  Total Protein Quantification
Resources
- Application Notes
  
  BioTek’s application notes present the latest-breaking scientific content covering the vast range of applications across our entire product line. Browse applications by topic, detection and product or use the simple keyword search to find the application notes that will facilitate your research.
- Application Bulletins
  
  BioTek’s application bulletins provide brief, concise descriptions of timely applications enabled with BioTek instrumentation.
- Application Guides
  
  Many applications are straightforward and need no explanation, but best practices and tips & tricks are useful for many complex or esoteric workflows. BioTek’s application guides offer detailed information on a variety of topics.
- Scientific Publications
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- Scientific Posters
  BioTek’s application team has developed more than 170 scientific posters on their latest research highlighting the applications enabled with our life science instrumentation. Browse or search the topics or products of interest.
- Technical Notes
  
  Our Technical Notes illustrate product-specific capabilities and common applications for BioTek microplate instrumentation, and their implementation in common experimental design.
- Visual Abstracts
  BioTek’s Visual Abstracts are brief, animated presentations that describe the workflow of a single specific application. Imaging & microscopy-focused Visual Abstracts explain the Augmented Microscopy workflow for a wide range of assays, with key capture>process>analyze>publish concepts presented. Other Visual Abstracts describe the utility of BioTek’s liquid handling and multi-mode instruments in a variety of applications. Check back often for our latest additions to the Visual Abstract library!
- Webinars
  Our webinar archives are available for on-demand viewing - search to find a topic, application or product of interest. You can also register to be invited to future webinars here.
- White Papers
  Browse BioTek’s white papers for timely information on such important topics as Reactive Oxygen Species and 3D Cell culture.
- Citations
  
  Search the more than 3,000 scientific citations for BioTek’s products within our database to learn more about the applications for our life science instrumentation as they’re used around the world.
- Videos
  Here you will find a collection of videos found throughout our site. These videos can also be found on BioTek's YouTube channel.
- Upcoming Webinars
  Through our Webinar presentations, BioTek strives to share information and experiences about timely and important microplate-based applications. Sign up today and check back for future topics that may be of interest to you.
Service & Support
- Sales & Support Offices
  BioTek's highly trained sales and service professionals are experts in life science instrumentation. They will guide you through your decision process and help you select the proper equipment for your application, assist with installation in your lab, and train your staff on the use of the instrumentation.
- Field Applications Scientists
  BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software.
- Technical Support
  The BioTek Global Technical Support Center (GTS) is your Help Desk for all questions related to BioTek product use, maintenance, troubleshooting support and other frequently asked questions. Normal GTS hours are weekdays from 8:30 A.M. - 5:30 P.M Eastern Standard Time, excluding holidays.
- Global Service
  BioTek Instrument Service and Repair is available worldwide at several of our International Service Centers. Field Service is also available for many customers throughout the world.
- FAQ & Discussions
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- Customer Resource Center
  Access BioTek’s Customer Resource Center to search frequently asked questions and discussion groups for imaging & microscopy, detection, liquid handling, robotics and software. If you’re already a customer, login for full access to participate in peer-to-peer discussions, download user manuals and software, and view order or product information specific to you! To register for the first time, click Login and Not a Member to sign up and receive instant access. Having trouble? Watch this quick registration tutorial.
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About Us
- News
  BioTek Launches a Set of Quantitative Assay Apps to Simplify Detection-Based Applications
  October 31, 2019 - The new Quantitative Assay Apps from BioTek Instruments, Inc. provide convenience and simplicity for researchers performing four commonly used detection-based applications: absorbance-based BCA, Bradford, Lowry protein assays, and fluorescence-based DNA assays. These easy-to-use Apps are powered through Gen5™ Data Analysis Software and are compatible with BioTek microplate readers equipped with the relevant detection mode, including the popular Cytation™ and Synergy™ multi-mode readers. Read more
  
  Agilent Completes Acquisition of BioTek Instruments - Builds on Multi-Year Growth Strategy in Critical Cell Analysis Technology
  August 26, 2019 - SANTA CLARA, CA - Agilent Technologies Inc. (NYSE: A) today announced it has completed the acquisition of BioTek Instruments, a global leader in the design, manufacture and distribution of innovative life science instrumentation. Read more
  
  BioTek Recognized as 2019 ‘Vermont Business Growth Award’ Winner
  August 12, 2019 - BioTek Instruments will be recognized as a 2019 Vermont Business Growth Award winner at a ceremony to be held September 17, 2019 at the Hilton Hotel Burlington, in Burlington, VT. This award is presented annually by Vermont Business Magazine and KeyBank, acknowledging twenty-five Vermont businesses that have experienced the greatest growth over the past five years. Read more
  MORE NEWS
- Events
  SLAS 2020
  
  January 25 - 29, 2020
  
  Booth #505
  
  San Diego Convention Center
  BioTek instrumentation will be on display by BioTek U.S.
  
  San Diego, CA
  United States
  
  The 27th Symposium on Recent Advances in Cellular and Molecular Biology
  
  February 5 - 7, 2020
  
  Booth #E
  
  YOHO Beach Resort
  BioTek instrumentation will be on display by BioTek Taiwan.
  
  Pingtung County
  Taiwan
  
  LS2 Annual Meeting 2020
  
  February 13 - 14, 2020
  
  University of Zurich, Campus Irchel
  BioTek instrumentation will be on display by BioTek Switzerland.
  
  Zurich
  Switzerland
  
  MORE events
- Customer Spotlights
  We are dedicated to developing and supporting instrumentation that enables a broad range of applications for scientists around the world. We can tell you about how BioTek instrumentation can benefit your research, but we’d rather you hear it directly from our customers! Browse the Customer Spotlights below to see how BioTek is helping to make their research possible.
- Awards
  See some of the many awards that BioTek and our products have received over the years.
- History
  BioTek is a family-run organization with origins dating back to 1968. This year we celebrate 50 years of Passion & Innovation. Click to view a historical timeline of BioTek's milestones since entering the life science market.
- Partners
  As a prospective partner, BioTek can help you achieve your sales and market penetration by providing a unique blend of products, service, support, training, co-marketing and quality assurance designed to meet your customers’ requirements while you remain focused on your core business.
- TekTalk Newsletter
  Review past issues of TekTalk, BioTek's complimentary life science instrumentation e-newsletter. If you are not currently subscribed, you may sign up now to begin receiving issues as they are released. Our current distribution is 6 issues/year.
- Blog
  Visit our blog, Scientifically Speaking, to read what our scientists and instrument experts have to share.
- Careers
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- Quality & Compliance
  BioTek is committed to maintaining the high quality of our products and services. All of our products are designed and manufactured in the USA at our Headquarters in Winooski, Vermont. Our continual improvement process includes soliciting customer input on quality and product features, and then designing, validating, manufacturing, and testing products to ensure performance and reliability.
- Contact Us
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SEARCH

Resources - Visual Abstracts

Automatic Transfection Efficiency Calculation in Microplates

Related Products: Cytation 1, Cytation 5, Lionheart FX

November 13, 2018

The family of GFP photoproteins are useful tools for live cell analysis. The BacMam baculovirus is an easy to use vector to transiently transfect cells with GFP. The efficiency of the transfection can be determined by counting cells expressing green fluorescence relative to the total number of cells.