Products
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- Imaging & Microscopy
  Automated Cell Imagers
  
  Lionheart FX Automated Microscope
  Lionheart LX Automated Microscope
  Confocal Imaging Reader
  
  Cytation C10 Confocal Imaging Reader
  Cell Imaging Multi-Mode Readers
  
  Cytation 7 Cell Imaging Multi-Mode Reader
  Cytation 5 Cell Imaging Multi-Mode Reader
  Cytation 1 Cell Imaging Multi-Mode Reader
  Multi-Plate Live Cell Imaging System
  
  BioSpa Live Cell Analysis System
  Peripherals
  
  AutoScratch Wound Making Tool
  Gas Controller
  Dual Reagent Injector Module
  Product
  Comparison Charts
  
  Imaging & Microscopy
  
  Gen5 Edition Comparison
- Detection
  Multi-Mode Microplate Readers
  
  Synergy Neo2 Hybrid Multi-Mode Reader
  Cytation Hybrid Multi-Mode Reader
  Synergy H1 Hybrid Multi-Mode Reader
  Synergy HTX Multi-Mode Reader
  Synergy LX Multi-Mode Reader
  Multi-Plate Absorbance Reader
  
  LogPhase 600 Microbiology Reader
  Absorbance Microplate Readers
  
  Epoch 2 Microplate Spectrophotometer
  Epoch Microplate Spectrophotometer
  800 TS Absorbance Reader
  Peripherals
  
  Gas Controller
  Take3 Plate
  Dual Reagent Injector Module
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  Multi-Mode Readers
  
  Absorbance Readers
  
  Microplate Spectrophotometers
  
  Gen5 Edition Comparison
- Liquid Handling
  Multi-Mode Washer Dispensers
  
  EL406 Washer Dispenser
  MultiFlo FX Multi-Mode Dispenser
  Microplate Washers
  
  405 TS Washer
  405 LS Washer
  ELx405 Select Deep Well Washer
  50 TS Washer
  Microplate Dispensers
  
  MultiFlo FX Multi-Mode Dispenser
  MicroFill Dispenser
  Product Comparison Charts
  
  Microplate Washers
  
  Microplate Dispensers
- Software
  Software
  
  Gen6 Data Analysis Software
  Gen5 Software Features for Imaging & Microscopy
  Gen5 Software Features for Detection
  Liquid Handling Control Software
  Software Apps
  
  Scratch Assay App
  LogPhase 600 App
  Product Comparison Charts
  
  Gen5 Edition Comparison
- Robotics
  Robotics
  
  BioSpa 8 Automated Incubator
  BioStack Microplate Stacker
  BenchCel Microplate Handler
  Product Comparison Charts
  
  BioStack Configuration Comparison
- Buy Cell Culture Microplates
  Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly.
  
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- Technologies
  Multi-Mode Detection Technologies
  
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  Imager & Microplate Reader Technologies
  
  4-Zone Incubation and Condensation Control
  Augmented Microscopy
  Confocal Technology
  Hybrid Technology
  Laser Autofocus
  Peltier Cooling Module
  Variable Bandwidth Monochromators
  Liquid Handling Technologies
  
  Automated, Gentle Media Exchange
  Dual-Action Manifold
  Parallel Dispense
  Random Access Dispense
  Ultrasonic Advantage
  Verify Technology
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Peripherals
- Peripherals
  BioTek’s peripheral products help to expand your BioTek instrument applications and improve workflow efficiency. From gas controllers to micro-volume plates, we have the peripherals to help advance your research.
- Accessories
  To complete or enhance your BioTek imager, microplate reader, liquid handling or robotics system, we offer a wide range of accessories, from tubing and bottles to imaging filter cubes. Search our Accessory database for Accessories compatible with your instrument. Search by Keyword, Part #, Product or Accessory Type.
Applications
- Cancer Research
  The field of cancer research spans diverse disciplines and requires a multipronged approach to understand the complex factors driving cancer formation and progression, as well as for the development of effective therapeutics.
  
  BioTek instruments provide versatile platforms and integrated solutions for conducting a broad range of cancer research studies.
- Cell Counting
  The ability to accurately determine cell number is an important aspect of a broad range of applications, including setting up and optimizing cell-based assays, normalizing data across samples, and conducting cell proliferation assays.
  
  The Agilent BioTek Lionheart FX automated microscope and Agilent BioTek Cytation cell imaging multi-mode readers utilize label-free and fluorescence imaging modes, along with powerful Agilent BioTek Gen5 image analysis tools, to deliver efficient and effective automated cell counting.
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- Cell Migration and Invasion
  
  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Endotoxin Analysis
  Bacterial endotoxins are lipopolysaccharides (LPS) generally found in the outer membrane of gram-negative bacteria, such as E.coli, and are widely present in our environment. Because of the pyrogenic nature of endotoxins, with human effects from fever to hemorrhagic stroke, FDA regulations require that raw materials (e.g. water) used in parenteral drugs be tested for endotoxin levels.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation and Lionheart FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5 image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- Microbiology
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
Resources
- Application Notes
  
  Our application notes present the latest-breaking scientific content covering the vast range of applications across our entire product line. Browse applications by topic, detection and product or use the simple keyword search to find the application notes that will facilitate your research.
- Application Bulletins
  
  Our application bulletins provide brief, concise descriptions of timely applications enabled with BioTek instrumentation.
- Application Guides
  
  Many applications are straightforward and need no explanation, but best practices and tips & tricks are useful for many complex or esoteric workflows. Our application guides offer detailed information on a variety of topics.
- Scientific Publications
  Search or browse our database to find scientific articles submitted by BioTek’s applications scientists and published in leading journals.
- Technical Notes
  
  Our Technical Notes illustrate product-specific capabilities and common applications for BioTek microplate instrumentation, and their implementation in common experimental design.
- Visual Abstracts
  Our Visual Abstracts are brief, animated presentations that describe the workflow of a single specific application. Imaging & microscopy-focused Visual Abstracts explain the Augmented Microscopy workflow for a wide range of assays, with key capture>process>analyze>publish concepts presented. Other Visual Abstracts describe the utility of BioTek’s liquid handling and multi-mode instruments in a variety of applications. Check back often for our latest additions to the Visual Abstract library!
- White Papers
  Browse our White Papers for timely information on such important topics as Reactive Oxygen Species and 3D Cell culture.
- Videos
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  Our webinar archives are available for on-demand viewing - search to find a topic, application or product of interest. You can also register to be invited to future webinars here.
Service & Support
- Field Applications Scientists
  BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software.
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- Customer Resource Center
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- TekTalk Newsletter
  Review past issues of TekTalk, BioTek's complimentary life science instrumentation e-newsletter. Our current distribution is 6 issues/year.
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Resources - Visual Abstracts

Automatic Transfection Efficiency Calculation in Microplates

Related Products: Cytation 1, Cytation 5, Lionheart FX

November 13, 2018

The family of GFP photoproteins are useful tools for live cell analysis. The BacMam baculovirus is an easy to use vector to transiently transfect cells with GFP. The efficiency of the transfection can be determined by counting cells expressing green fluorescence relative to the total number of cells.

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