Automated Colony Formation Assay using Cytation 7 Upright Color Brightfield Imaging

The colony formation assay evaluates the proliferative capacity of a single cell and provides valuable insight into cancer cell behavior and treatment response. Conventional analysis involves scoring and quantifying colonies in each well of a multi-well format manually, with limited throughput. Here we present an automated method for conducting and analyzing the colony formation assay in 6- to 96-well microplate formats, using upright color brightfield imaging with the Cytation 7 Cell Imaging Multi-Mode Reader.