Resources - Technical Notes

MicroFlo Select and MicroFill Reagent Dispensers Comparison - Flow Rate and Velocity Differences between Peristaltic and Syringe Pump Dispensers

Download

Related Products: MicroFill

October 13, 2008

 

 Flow rate is defined as the volume of fluid which passes through a dispense tip per unit of time. Velocity is the rate of change of fluid position.

Below find comparison tables for flow rates and velocities using BioTek’s MicroFlo Select and MicroFill Dispensers. This information will be useful when determining the most appropriate dispense technology and setting for a particular reagent and/or application.

 

MicroFlo Select Dispenser (peristaltic pump) - 1x8 manifold
Cassette Setting Flow Rate
(μL/tip/sec)
Velocity
(mm/tip/sec)
1 μL, 0.0075” tip Low 56 1754
Med 60 2105
High 64 2245
5 μL, 0.014” tip Low 120 1208
Med 140 1410
High 160 1611
10 μL, 0.014” tip Low 140 1410
Med 160 1611
High 180 1812

96-well microplate = 1 tip/well
384-well microplate = 1 tip/well

MicroFill Dispenser (syringe pump) – 1x16 manifold
Setting 0.020” Flow Rate
(μL/tip/sec)
Velocity
(mm/tip/sec)
1 225 1133
2 300 1510
3 375 1888
4 450 2265
5 500 2517

96-well microplate = 2 tips/well
384-well microplate = 1 tip/well

 

Contact BioTek’s Technical Assistance Center with questions.

link