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Sample Files

Solution ELISA Prozone and Sensitivity assay

June 05, 2013

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Related Products: Gen5 for Detection, Synergy Neo2

Related App Note: Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products


Gen5 version required: 2.0 or higher


Gen5 was used to determine luminescence signal in a solution ELISA assay for evaluation of the sensitivity and prozone effect of the positive control used during immunogenicity testing for anti-drug antibodies. The positive control was serial diluted to evaluate the lowest concentration meeting the acceptance criteria for intra-and inter-assay precision and the ability to detect ~5% false positives among samples tested. The sample file contains the read parameters used for this experiment. Data was collected and exported to Microsoft Excel for further statistical analysis.

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