RiboGreen High Concentrations
November 27, 2012Download
Related Products: Gen5 for Detection
Gen5 version required: 1.0 or higher
Basis for the Assay:
RiboGreen stain binds to RNA. With binding the stain’s fluorescence increases markedly. The stain is not necessarily specific for RNA, but will also bind ssDNA and dsDNA molecules. The fluorescence intensity is directly proportional to the nucleic concentration present in the sample.
The protocol calls for a fluorescence measurement using a 485/20 excitation filter and a 528/20 emission filter.
The experiment data file contains all of the fluorescence determinations for a RiboGreen RNA determination.
Plate configuration assumes that samples will be run in duplicate. Included is a RNA standard curve (0 to 1,000 ng/ml). The plate includes 5 standard concentrations, but has no blanks or controls wells. Thus, 43 samples can be run on a microplate in duplicate, in addition to the standard curve.
The report is configured to provide the (1) raw fluorescence, (2) standard curve, and (3) calculated protein concentrations and statistics of unknown samples.