Quantitative ELISA Glow Luminescence

Gen5 version required: 1.0 or higher

Basis for the Assay:

ELISA assays are an extremely common way to detect biomolecules in complex samples. Most ELISA assays use absorbance as a detection method. In some cases, when the concentrations to be measured are extremely low, luminescence provides more sensitivity and is used instead of absorbance. The assay principle remains exactly the same, the only difference being the use of a luminescent substrate instead of a colorimetric one at the end of the process.

This protocol executes a 1-second read per well in luminescence mode. Results available at the end of the process are: raw data, blanked data, standard curve, calculated sample concentration.

The experiment data file does not contain any data.

Plate Configuration:

Plate configuration includes a blank well, a standard curve (0.5 to 5000 pg/ml) based on 6 standards, and 41 samples, all in duplicate.

Report:

The report provides:
1. Plate layout and calculated concentrations in a matrix format
2. Standard curve
3. Statistics on duplicates