Gen5 version required: 1.0 or higher
Basis for the Assay:
Enzyme linked immunosorbent assays (ELISA) have been used to quantitate a wide range of compounds and pathogens for almost 40 years. One of the most commonly used enzyme conjugates is horseradish peroxidase (HRP). QuantaBlu™ serves as a substrate for HRP. Unreacted Quanta Blu is nonfluorescent, but in the presence of hydrogen peroxide (H2O2) and HRP it is converted to a fluorescent product, which has excitation and emission maxima of 325 nm and 420 nm respectively. ELISA assays that employ an HRP conjugate can be used with Quanta Blu substrate.
The protocol calls for a fluorescence measurement using a (320/20) excitation filter and a (420/50) emission filter.
The experiment data file does not contain any data.
Plate configuration assumes that samples will be run in duplicate. The plate includes two blank wells, and no controls wells. A standard curve based on the analyte in question is required.
The report is configured to provide the (1) Blanked fluorescence.