Qualitative ELISA Glow Luminescence
November 29, 2012Download
Related Products: Gen5 for Detection
Gen5 version required: 1.0 or higher
Basis for the Assay:
ELISA assays are an extremely common way to detect biomolecules in complex samples. Most ELISA assays use absorbance as a detection method. In some cases, when the concentrations to be measured are extremely low, luminescence provides more sensitivity and is used instead of absorbance. The assay principle remains exactly the same, the only difference being the use of a luminescent substrate instead of a colorimetric one at the end of the process.
This protocol executes a 1-second read per well in luminescence mode. Results available at the end of the process are: raw data, blanked data, and qualitative interpretation based on a cut-off calculation.
The experiment data file does not contain any data.
Plate configuration includes a blank well, positive control, negative control and 45 samples, all in duplicate.
The report provides:
1. Plate layout and qualitative interpretation (Positive/Negative) in a matrix format
2. Statistics on sample duplicates.