Gen5 version required: 1.0 or higher
Basis for the Assay:
The presence of trace amounts of phenol can denature proteins and inhibit enzymatic reactions. The A260/A270 ratio for nucleic acid sample should be greater than 1.0. Ratios less than 1.0 suggest the presence of trace amounts of phenol.
The procedure steps include a reading at 260 nm and 270 nm. Background absorbance of the microplate is removed by subtraction of a blank well. Using a Transformation the A260/A270 ratio is calculated. Wells with a ratio value of less than 1.0 are considered to be positive for the presence of phenol. This is expressed in a Cutoff data set.
The experiment data file does not contain any data
The plate layout places one blank in position A1. The rest of the wells contain 95 individual samples.
Plate Report is configured to provide the (1) blanked 260 nm absorbance; (2) blanked 270 nm absorbance and (3) Calculated 260/270 ratio. A Plate report using a cutoff of 1.0 on the 260/270 ratio indicating the possible presence (POS) or absence (NEG) of EDTA is also reported.