Gen5 version required: 1.0 or higher
Basis for the Assay:
Purification procedures can often lead to the formation of insoluble particulates that remain after centrifugation. Absorbance values greater than 0.000 OD (after blanking) indicate the presence of particulates in nucleic acid samples.
The protocol calls for an absorbance measurement at 320 nm. Background absorbance of the microplate is removed by subtraction of a blank well. Wells with a value of 0.010 or greater are considered to be positive for the presence of particulates. This is expressed in a Cutoff data set.
The experiment file does not contain any data.
The plate layout places one blank in position A1. The rest of the wells contain 95 individual samples.
The Report is configured to provide the (1) blanked 320 nm absorbance; (2) A Plate report using a cutoff of 0.010 indicating the possible presence (POS) or absence (NEG) of particulate is also reported.