Parallel Line Analysis applied to fluorescence polarization data from a Predictor hERG assay

Gen5 version required: 2.0 or higher

This sample file shows a dose comparison of E410 with astemizole, a known hERG binding molecule. Using Gen5’s Parallel Line Analysis (PLA) feature, the relative potency of E410 was calculated to be 0.0683 of astemizole. While this indicates that this compound is less likely to bind than astemizole, the fact that it displaces the tracer indicates that it is a hERG-binding molecule.

Parallel Line Analysis (PLA) and EC50 calculations performed in Gen5