Gen5 version required: 1.0 or higher
Basis for the Assay:
Reporter genes, such as luciferase, are important tools for studying gene expression. The use of reporter genes allows the in vitro and in vivo measurement of gene expression from virtually any endogenous genetic control element. Luciferase enzyme and its subsequent luminescent reaction is often the gene reporter of choice for many experimental conditions. This assay requires automated injection and luminescence detection. “Flash” assays are typically more sensitive than “Glow” assays (see Glow Luciferase Assay), but provide a lower throughput.
This protocol executes the following process:
1. Injection of the trigger reagent (100 μl)
2. Wait two seconds 3. Read sample for 5 seconds
The experiment data file does not contain any data.
Plate configuration contains samples only. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, blanks, controls).
The report provides:
1. A summary of the procedure
2. The plate layout
3. Statistics on sample replicates