Long Kinetic Luminescence
November 29, 2012Download
Related Products: Gen5 for Detection
Basis for the Assay:
Cell-based assays often require following the change in signal over some period of time. This assay performs a plate-mode kinetic measurement with temperature control.
This protocol executes the following process:
1. Bring reading chamber to 37ºC before starting the read process
2. Measure luminescence kinetically for 2 hours with a 5-minute interval, each measurement is a 1 second integration
Results available at the end of the process are: raw data, kinetic curves, kinetic analysis: Maximum slope (Max V).
The experiment data file does not contain any data.
Plate configuration contains samples only, in duplicate. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, controls).
The report provides:
1. Matrix of kinetic curves
2. Well zoom examples
3. Statistics on samples