HDAC-Glo I/II Assay Reading Parameters and Data Analysis for Assay Optimization and Screening

Related App Note: Assessment of HDAC1 Inhibition Using an Automated, Bioluminescent Histone Deacetylase I/II Assay

Gen5 version required: 2.0 or higher

Gen5™ software is used to compute signal:background ratios for assay optimization and validation experiments such as enzyme titration and Z’-factor. % inhibition is also calculated for use with compound inhibitor screening and secondary dose response tests. A linear enzyme titration curve and compound dose response curve is also included to illustrate how these curves can aid in data analysis.