Sample Files

Glucose (Amplex Red)

November 27, 2012

Download

Related Products: Gen5 for Detection



Gen5 version required: 1.0 or higher



Basis for the Assay:

Cholesterol oxidase reacts with D-glucose to form D-gluconolactone and hydrogen peroxide (H2O2). Amplex red reagent and H2O2 are converted to resorufin and water in a one-to-one stiochiometry by horseradish peroxidase. Resorufin is a highly fluorescent compound with absorption maxima of 571 nm and a peak emission wavelength of 585 nm. The amount of fluorescence produced is directly proportional to the amount of D-glucose present in the sample.

The protocol calls for a fluorescence measurement using a 540/35 excitation filter and a 590/20 emission filter.

The experiment data file does not contain any data.

Plate Configuration:


Plate configuration assumes that samples will be run in duplicate. Included is a standard curve of D-glucose concentration (0 to 125 μM) as defined by Invitrogen’s Glucose/glucose oxidase Assay kit. The plate includes 8 standard concentrations, but no blank or controls wells. Thus, 40 samples can be run on a microplate in duplicate, in addition to the standard curve.

Report:

The report is configured to provide the (1) raw fluorescence, (2) standard curve, (3) calculated cholesterol concentrations and statistics of unknown samples.

link