Sample Files

Fast Kinetic Luminescence

November 29, 2012

Download

Related Products: Gen5 for Detection



Gen5 version required: 1.0 or higher



Basis for the Assay:

Some luminescent assays have extremely fast kinetic signals, and it is sometimes useful to measure the kinetic of the signal after injection rather than just integrate it for a few seconds. The obtained kinetic curve can provide more detailed information than an endpoint integration. This assay requires automated injection and luminescence detection.

This protocol executes the following process: 1. Injection of the trigger reagent (100 μl) 2. Measure luminescence kinetically for 10 seconds with a 20 ms interval (50 data points/second) Results available at the end of the process are: raw data, kinetic curves, kinetic analysis (Min/Max analysis).

The experiment data file does not contain any data.

Plate Configuration:

Plate configuration contains only samples, in duplicate. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, controls).

Report:


The report provides:
1. Matrix of kinetic curves
2. Well zoom examples
3. Statistics on samples

link