Fast Kinetic Luminescence
November 29, 2012Download
Related Products: Gen5 for Detection
Gen5 version required: 1.0 or higher
Basis for the Assay:
Some luminescent assays have extremely fast kinetic signals, and it is sometimes useful to measure the kinetic of the signal after injection rather than just integrate it for a few seconds. The obtained kinetic curve can provide more detailed information than an endpoint integration. This assay requires automated injection and luminescence detection.
This protocol executes the following process: 1. Injection of the trigger reagent (100 μl) 2. Measure luminescence kinetically for 10 seconds with a 20 ms interval (50 data points/second) Results available at the end of the process are: raw data, kinetic curves, kinetic analysis (Min/Max analysis).
The experiment data file does not contain any data.
Plate configuration contains only samples, in duplicate. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, controls).
The report provides:
1. Matrix of kinetic curves
2. Well zoom examples
3. Statistics on samples