Gen5 version required: 1.0 or higher
Basis for the Assay:
FAM (fluorescein) is a fluorescent compound widely used for oligonucleotide labeling. Labeled oligonucleotides have been used as detection probes for real time PCR (RTPCR), as well as primers for DNA PCR reactions and DNA sequencing.
The protocol calls for a fluorescence measurement using a 485/20 excitation filter and a 528/20 emission filter.
The plate data file does not contain any data.
Plate configuration provides for samples to be run in duplicate. In addition, two blank wells are defined on the plate. In regards to data reduction, only blank well subtraction has been programmed.
The report is configured to provide the (1) raw fluorescence and (2) blanked fluorescence.