Gen5 version required: 1.0 or higher
Basis for the Assay:
Ethidium bromide is a DNA binding fluorescent dye the exhibits red fluorescence. Ethidium bromide stain intercalates into the minor groove of dsDNA, showing little or no sequence preferences. Binding to DNA results is an approximate 10-fold enhancement in fluorescence.
The protocol calls for a fluorescence measurement using a 540/35 excitation filter and a 620/40 emission filter.
The plate data file does not contain any data.
Plate configuration includes two blank wells and 94 unknown samples. The only data reduction performed is blank subtraction.
The report is configured to provide (1) blanked fluorescence.