Gen5 version required: 1.0 or higher
Basis for the Assay:
α-Amylase is the catalytic enzyme responsible for the hydrolysis of starch into smaller components such as maltose and dextrins. Quantitation of α-Amylase is important in the diagnosis of pancreatitis and diabetes, as well as, a number of different industrial applications. The test is based on the digestion of a starch substrate that has been labeled with BODIPT FL dye to such an extent that the fluorescence is quenched. When digestion of the substrate by the enzyme occurs the quenching is relieved, resulting in the release of fluorescent product.
The protocol calls for a fluorescence measurement using a 485/20 excitation filter and a 528/20 emission filter.
The experiment data file does not contain any data.
Plate configuration assumes that samples will be run in duplicate. The plate includes six different concentrations of standards ranging from 0 to 200 mU/ml. Thus, 42 samples can be run.
The report is configured to provide the (1) raw fluorescence, (2) standard curve, and (3) Amylase concentrations of unknown samples.