Sample Files

EDTA Inhibition Assay

November 20, 2012


Related Products: Gen5 for Detection

Gen5 version required: 1.0 or higher

Basis for the Assay:

The presence of EDTA inhibits divalent ion (e.g. Mg++, Mn++) dependent reactions. The A260/A230 ratio for pure DNA is approximately 2.0, values less than 2.0 suggest the presence of EDTA contamination.

The protocol calls for an absorbance measurement at 230 nm and 260 nm. Background absorbance of the microplate is removed by subtraction of a blank well. A transformation is used to calculate the A260/A230 ratio. Wells with a ratio value of less than 2.0 are considered to be positive for the presence of EDTA.This is expressed in a Cutoff data set.

The experiment data file does not contain any data.

Plate Configuration:

The plate layout places one blank located in position A1. The rest of the wells contain 95 individual samples.


Plate Report is configured to provide the (1) blanked 230 nm absorbance; (2) blanked 260 nm absorbance and (3) Calculated 260/230 ratio. A Plate report using a cutoff of 2.0 on the 230/260 ratio indicating the possible presence (POS) or absence (NEG) of EDTA is also reported.