Gen5 version required: 1.0 or higher
Basis for the Assay:
Accurate determination of DNA concentration in a sample is important for many different procedures in molecular biology. Luminescent-based detection of dsDNA is a sensitive and specific way to detect linear dsDNA including PCR fragments. Plasmid and chromosomal DNA can be quantitated following linearization. The measurement is based upon a series of coupled enzymatic reactions that produce a light signal proportional to the amount of linear DNA in a sample. This assay requires automated injection and luminescence detection. Protocol name: dsDNA Quantitation.prt (Luminescence Reader with injectors) This protocol executes the following process: 1. Injection of the trigger reagent (100 μl) 2. Wait three seconds 3. Read sample for 1 second Results available at the end of the process are: raw data, blanked data, and the calculated DNA concentration of samples based on the standard curve.
The experiment data file does not contain any data.
Plate configuration provides for samples to be run in duplicate. Included are five standards, a blank, and 42 samples, in duplicate.
The report provides:
- The plate layout, Well IDs, and blanked results
- Standard curve with curve fit information
- Statistics on blanks, standards and samples