Sample Files

dsDNA Quantitation

November 27, 2012

Download

Related Products: Gen5 for Detection



Gen5 version required: 1.0 or higher



Basis for the Assay:

Accurate determination of DNA concentration in a sample is important for many different procedures in molecular biology. Luminescent-based detection of dsDNA is a sensitive and specific way to detect linear dsDNA including PCR fragments. Plasmid and chromosomal DNA can be quantitated following linearization. The measurement is based upon a series of coupled enzymatic reactions that produce a light signal proportional to the amount of linear DNA in a sample. This assay requires automated injection and luminescence detection. Protocol name: dsDNA Quantitation.prt (Luminescence Reader with injectors) This protocol executes the following process: 1. Injection of the trigger reagent (100 μl) 2. Wait three seconds 3. Read sample for 1 second Results available at the end of the process are: raw data, blanked data, and the calculated DNA concentration of samples based on the standard curve.

The experiment data file does not contain any data.

Plate Configuration:

Plate configuration provides for samples to be run in duplicate. Included are five standards, a blank, and 42 samples, in duplicate.

Report:

The report provides:
  1. The plate layout, Well IDs, and blanked results
  2. Standard curve with curve fit information
  3. Statistics on blanks, standards and samples
link