Sample Files

DAPI

November 27, 2012

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Related Products: Gen5 for Detection



Gen5 version required: 1.0 or higher



Basis for the Assay:

DAPI (4’,6’-diamidino-2-phenylindole) is a DNA binding fluorescent dye the exhibits blue fluorescence. DAPI stain associates in the minor groove of dsDNA, preferentially binding to AT-base clusters. Binding to DNA results is an approximate 20-fold enhancement in fluoresescence.

The protocol calls for a fluorescence measurement using a 360/40 excitation filter and a 460/40 emission filter.

The plate data file does not contain any data.

Plate Configuration:

Plate configuration includes two blank wells and 94 unknown samples. The only data reduction performed is blank subtraction.

Report:

The report is configured to provide the (1) raw fluorescence and (2) blanked fluorescence.

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