Gen5 version required: 1.0 or higher
Basis for the Assay:
DAPI (4’,6’-diamidino-2-phenylindole) is a DNA binding fluorescent dye the exhibits blue fluorescence. DAPI stain associates in the minor groove of dsDNA, preferentially binding to AT-base clusters. Binding to DNA results is an approximate 20-fold enhancement in fluoresescence.
The protocol calls for a fluorescence measurement using a 360/40 excitation filter and a 460/40 emission filter.
The plate data file does not contain any data.
Plate configuration includes two blank wells and 94 unknown samples. The only data reduction performed is blank subtraction.
The report is configured to provide the (1) raw fluorescence and (2) blanked fluorescence.