CyQuant Cell Proliferation Assay
November 27, 2012Download
Related Products: Gen5 for Detection
Gen5 version required: 1.0 or higher
Basis for the Assay:
CyQuant Cell Proliferation Assay is a means to directly quantitate the density of cells in culture. CyQuant Cell Proliferation assay uses a proprietary dye, CyQuant GR, which becomes fluorescent when bound to cellular nucleic acids. Cells are lysed by the addition of lysis buffer containing the dye and the samples are quantitated directly. A reference standard curve using specific numbers of cells can be created that allows converting fluorescence into cell number. Alternatively cellular DNA can be directly quantitated using a DNA only standard curve.
The protocol calls for a fluorescence measurement using a 485/20 excitation filter and a 528/20 emission filter.
The experiment data file does not contain any data.
Plate configuration assumes that lysed and RNase treated samples will be run in duplicate. The plate includes nine different DNA concentrations of standards ranging from 0 to 1000 ng/mL. Thus, 39 samples can be run in duplicate.
The report is configured to provide the (1) raw fluorescence, (2) standard curve, and (3) DNA concentrations of unknown samples.