Critical micelle concentration determination using fluorescence polarization

 
Gen5 version required: 2.0 or higher

The sample file provides assay reading parameters for a simultaneous parallel and perpendicular measurements for green FP determination. The plate map included concentrations for 4 separate determination using different salt concentrations. As part of the Excel report, the CMC values for each of the 4 curves are mathematically determined from the information provided by the curve fit parameters. This Excel calculation uses a mathematical determination of the slope of the rapidly changing portion of a 4-parameter fit curve to create a tangential line that intersects the lower concentration “A” parameter.