Sample Files

Bradford Protein Assay

November 20, 2012


Related Products: Gen5 for Detection

Gen5 version required: 1.0 or higher

Basis for the Assay:

Quantitation of total protein content is a measurement common to many applications in basic science and clinical research. The basis for this assay is the binding of Coomassie Brilliant Blue G-250 to protein with a resultant shift in the absorbance maximum from 465 to 595. Absorbance at 595 nm is used to quantitate protein content.

The protocol calls for an endpoint absorbance measurement at 595 nm. Plate map with a standard curve from 0 to 100 μg/ml with samples and standards in duplicate. Linear regression curve fit is used to determine unknown concentrations

The experiment file contains the absorbance determination, which is used to plot the standard curve.

Plate Configuration:

The plate layout includes standards in duplicate, from 0 to 100 μg/ml, in well positions A1 through B5. Samples are also in duplicate, for a total of 43 samples in this example.


The report includes the standard curve, and it’s equation parameters and correlation coefficients. A matrix report is configured to provide the (1) 595 nm absorbance and (2) calculated protein concentrations. A column report contains the statistics for standard and sample concentrations.