Sample Files

ATP Glow

November 27, 2012

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Related Products: Gen5 for Detection



Gen5 version required: 1.0 or higher



Basis for the Assay:

All living things utilize ATP as a means for storing metabolic energy. Because of this, the detection and quantitation of ATP can be used as a means to detect and/or quantitate microorganisms such as bacteria and somatic cells. A very common assay relies on the ATPdependence of the firefly luciferase reaction to detect live organisms. “Glow” assays are typically less sensitive than “Flash” assays (see Flash ATP Assay), but don’t require automated injection and have a higher throughput.

This protocol is an end-point luminescence measurement integrating each well for 1 second.

The experiment data file does not contain any data.

Plate Configuration:


Plate configuration contains samples in duplicate and a Blank well in duplicate. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, controls).

Report:

The report provides:
  1. The plate layout, Well IDs, and blanked results
  2. Statistics on sample replicates
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