Gen5 version required: 1.0 or higher
Basis for the Assay:
All living things utilize ATP as a means for storing metabolic energy. Because of this, the detection and quantitation of ATP can be used as a means to detect and/or quantitate microorganisms such as bacteria and somatic cells. A very common assay relies on the ATPdependence of the firefly luciferase reaction to detect live organisms. This assay requires automated injection and luminescence detection. “Flash” assays are typically more sensitive than “Glow” assays (see Glow ATP Assay), but provide a lower throughput.
This protocol automates the following process: 1. Injection of the trigger reagent (100 μl) 2. Wait two seconds 3. Read sample for 5 seconds
The experiment data file does not contain any data.
Plate configuration contains samples only, in duplicate. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, blanks, controls).
The report provides: 1. A summary of the procedure 2. The plate layout 3. Statistics on sample replicates