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Sample Files

AlphaLISA Drug Tolerance

June 05, 2013


Related Products: Gen5 for Detection, Synergy Neo2

red arrow Related App Note: Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products

Gen5 version required: 2.0 or higher

Gen5 was used to determine the AlphaLISA signal for analysis of the extent to which circulating drug interferes with assay performance due to competition between circulating drug and the assay antigen for ADAs. The sample file contains the read parameters used for this assay. The protocol contains an absorbance read step in an unused well to allow for a delay prior to reading the Alpha signal. Data was collected for statistical analysis after export to Microsoft Excel.