Gen5 version required: 1.0 or higher
Basis for the Assay:
Alexa Fluor® 488 is a member of the Alexa Fluor series of fluorescent dyes manufactured
and sold by Molecular Probes (Invitrogen). These dyes are reported to be brighter, have
better photostability, have a greater pH range and water solubility, as well as have
improved labeling chemistry than many conventional fluorescent compounds. Alexa Fluor
488 would be a replacement for fluorescein in regards to excitation and emission
The protocol calls for a fluorescence measurement using a 485/20 excitation filter and a
528/20 emission filter.
The experiment data file does not contain any data.
Plate configuration does not provide any sample or standard information. It may have to be
updated with more specific information (e.g., standards to generate a calibration curve,
The report is configured to provide the raw fluorescence.