Resources - Scientific Posters

Automation of a Microplate Cell-based Assay to Measure Activity of the Histamine H1 G-protein-coupled Receptor Using A Novel 3-D Cell Culture Technique

Download

Related Products: Precision

January 22, 2010

 

Authors: Brad Larson, Peter Banks, BioTek Instruments, Inc., Winooski, VT; Diana Berry, Brad Justice, Global Cell Solutions Inc., Charlottesville, VA, USA; Ronald Herzig, Invitrogen Corporation, Madison, WI, USA


Global Cell Solutions     Invitrogen

 

Cell-based assays using recombinant drug targets expressed in immortalized cell lines are today used more frequently than their biochemical counterparts in drug screening. Current cell culture methods use two-dimensional (2-D) techniques where cells attach to the microplate surface in a single monolayer. In order to maintain cell lines, complicated and time-consuming methods are used to trypsinize cells off of their growth surface, deactivate and remove the trypsin, split, and then replace cells into a new flask. This becomes increasingly diffi cult as scale-up is required for primary and secondary screens.

 

 
link

We use cookies to provide visitors of our website the best possible experience. If you continue without changing your browser settings, we'll assume you are happy to receive all cookies on the BioTek website. To learn more how we use cookies or how to block cookies, please visit our cookie policy.