Resources - Webinars

A New Look at Cell-based Assays in a Multi-Mode Microplate Reader using Fluorescence Microscopy

Related Products: Cytation 1

July 01, 2013

Presenters: Caleb Foster and Paul Held, BioTek Instruments Inc.

Multi-mode microplate readers are a useful tool for cell-based assays. These instruments use PMT-based optics to provide a cell population-averaged assay signal relative to assay controls that can quantify biomolecule expression, activation, inhibition and post-translational modulation. Fluorescence microscopy allows the analysis of sub-populations of cells or even analysis of a single cell, depending on the magnification used.

In this webinar we will demonstrate this dual capability using the Cytation™ 3 Cell imaging Multi-Mode Reader, which incorporates automated digital widefield fluorescence microscopy to perform sub-population analysis of cells in microplates. In addition, the PMT-based optics are used in conjunction with fluorescence microscopy to enable both cell population and sub-population analysis. Examples presented will include quantification of transfection efficiency, target-based inhibition studies with accompanying cell viability determination and phenotypic assays of oxidative stress and hypoxia.
 

A New Look at Cell-based Assays in a Multi-Mode Microplate Reader using Fluorescence Microscopy

 

link

We use cookies to provide visitors of our website the best possible experience. If you continue without changing your browser settings, we'll assume you are happy to receive all cookies on the BioTek website. To learn more how we use cookies or how to block cookies, please visit our cookie policy.