Resources - Application Notes
Utility of Variable Bandwidth Monochromators for Quantification of Fluorescent Probes in Produced Effluent WaterDownload
Related Products: Synergy Neo2
July 01, 2015
Authors: Brad Larson and Peter Banks, BioTek Instruments, Inc., Winooski, VT, USA; Wilhelm Glomm, Sintef, Trondheim, Norway
One of the primary waste products during the separation of oil, gas and water is produced effluent water, which is typically a mixture of formation and injection process water containing oil, salts, chemicals, solids and trace metals. Monitoring of oil content in water streams from oil production and refining is critical to meet stringent regulations for returning produced water to the environment, and is regulated by the Environmental Protection Agency (EPA) in the US. As the current trend is to further reduce the level of crude oil released in produced water streams, more advanced methods are necessary to accurately detect minute traces of oil in purified samples; many times reaching the parts per million (ppm) or parts per billion (ppb) level.
A number of reference methods have historically been used by the industry to measure oil in produced water. These include infrared absorption, gravimetric, and gas chromatography and flame ionization detection (GC-FID) methods. However, none have been universally adopted, and all are subject to limitations. While infrared methods are easily used, and can be deployed in the field, they involve the use of solvents that have been shown to be carcinogenic or harmful to the environment. Gravimetric methods are inexpensive and also easy to use, but do not provide details as to the composition of the sample and are subject to evaporative constraints. GC-FID has none of the problems of the previous methods. Yet it also has not been widely accepted due to the need for sophisticated instrumentation and a high degree of training for potential operators.
Newer methods, which incorporate a fluorescent probe, can alleviate the limitations of each of the previously listed methods, and also delivers a high level of sensitivity. This type of probe can also be used to monitor other pollutants, provided that the dye molecule has a high partitioning coefficient into the contaminant. The probe, introduced into the crude oil, is diluted during the purification process. Upon completion, a sample can be easily monitored using microplate reader technology without the need for harmful solvents or complicated procedures. As new probes are evaluated for potential introduction into the monitoring process, it is important to first understand the excitation and emission spectra of the fluorescent molecule, and then be able to tune the optical system to deliver the highest degree of sensitivity suitable for detection of the fluorescent probe when heavily diluted.
Here we demonstrate the ability to record excitation and emission spectra for fluorescent probes used in produced effluent water using the monochromator-based detection system of a microplate reader. The spectra provide the ability to begin the optimization process of wavelength and bandwidth selection for both excitation and emission monochromators. Here we demonstrate optimization for two individual probes with either a narrow (23 nm) or wide (67 nm) Stokes shift. The flexibility of the monochromatorbased system also allows for multiple fluorescent probes, and therefore multiple potential pollutants in the final water stream to be assessed.
Materials and Methods
Fluorescent Contaminant Probes
Unknown purified fluorescent contaminant probes 201 and 204 were donated by Sintef (Trondheim, Norway). Stock concentrations of each probe were 1 mg/mL, or 1000 ppm, in 100% methanol.
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Creation of Fluorescent Probe Titration
A solution made up of 70% methanol/30% 0.2 M sodium hydroxide was created for dilution of each probe. Stock solutions were initially diluted from the original 1000 ppm concentration to 1000 ppb. A serial titration was then performed using a 1:2 dilution scheme, creating an 11-point titration series ranging from 1000-1 ppb, in addition to a diluent only blank control.
Spectral Scanning and Fluorescent Monochromatorbased Microplates Reads
A single well containing the 1000 ppb sample was used to perform an excitation and emission scan of the unknown fluorescent probe to determine the spectral characteristics. The entire titration range was then read multiple times using the variable bandwidth monochromators, while varying the excitation and emission settings, and bandwidth. The process was performed for each sample provided.
Signal:Noise Ratio and Limit of Detection Determination
The signal:noise (S:N) ratio was calculated using the following formula:
(RFUProbe – Avg RFUBlank)/Std DevBlank
Where RFUProbe is the fluorescent signal from wells containing the probe titration, Avg RFUBlank equals the average signal from wells containing diluent only, and Std DevBlank equals the standard deviation of the RFU values from the same blank wells. The limit of detection (LOD) is calculated as the lowest probe concentration yielding a S:N ratio of 3, or a blanked RFU value three times the standard deviation of the blank.
Results and Discussion
Fluorescent Probe Spectral Profile
The first step in the process was to accurately determine the excitation and emission spectra of the unknown sample 201. Spectral scan read steps were created in the Gen5 software, and carried out using 1 nm increments to completely capture the shape and intensity of both spectra.
Figure 1. Sample 201 Excitation and Emission Spectra.
Raw RFU values were captured at each wavelength tested. The values were then normalized by finding the wavelength yielding the highest RFU value, and dividing the other RFU values by the maximum. Percent maximum RFU (% Max RFU) values were then plotted for each included wavelength value to create the two plots seen in Figure 1. A Stokes shift of 23 nm was determined for the probe.
Sample 201 Monochromator Setting Optimization: On Peak Assessment
In order to detect the lowest levels of crude oil or other pollutants in a purified water sample, limits of detection for fluorescent probes are required to be at the single ppb level or below. Therefore, monochromator optimization was carried out to determine the most ideal excitation and emission setting, in addition to bandwidth value, to meet the established criteria.
The first step was to set the excitation and emission values in a read step at the previously determined spectral peaks for the probe (on peak). Multiple bandwidth values were evaluated from the minimum wavelength range of 3 nm to the maximum of 50 nm (Figure 2).
Figure 2. Sample 201 On Peak Variable Bandwidth Wavelength Coverage. Included wavelengths shown when excitation and emission bandwidths set to 3, 6, 9, 12, 15, or 20 nm. Graphs for 30, 40, and 50 nm bandwidths not shown - excessive wavelength overlap lead to crosstalk, with excitation light being captured by the emission monochromator.
Wells containing the probe concentrations, diluted as previously described, were then read in a 96-well black plate using 10 measurements per data point and a delay after plate movement of 100 milliseconds.
Figure 3. On Peak Sample 201 Calculated Signal:Noise Ratio Values. Excitation and emission set to determined peak values of 494 and 517 nm, respectively. S:N values incalculable for 30, 40, and 50 nm bandwidth settings.
The mean of diluent only samples was subtracted from probe containing well RFUs to create a blanked value. This number was then divided by the calculated standard deviation value from diluent wells to provide a S:N ratio for each concentration of probe tested. Average S:N values were then plotted using a log:log scale in relation to the known [ppb] (Figure 3).
As seen by the results in Figure 3, S:N ratios for all concentrations tested increase as bandwidth expands to include a larger wavelength range, up to a value of 12 nm. This is due to the increasing amount of light used to excite the fluorescent molecule, as well as the increased range of fluorescence captured during emission. There is also minimal crosstalk, leading to minimal background levels. However, when using bandwidth values larger than 12 nm, an increasing amount of crosstalk is captured, causing background fluorescence to increase and lowering S:N ratios. Eventually, with the use of bandwidth values of 30 nm and above, the background signal is so great that fluorescence signals can no longer be accurately quantified.
The results from Figure 3 are confirmed when examining the LOD values for the bandwidths tested (Table 1). The lowest LOD is seen when a bandwidth of 9 or 12 nm is selected. It is also apparent that increased background fluorescence begins to significantly affect the sensitivity of the read beginning at a bandwidth of 20 nm.
Table 1. Sample 201 On Peak Limit of Detection Values. Excitation and emission values set at 494 nm and 517 nm, respectively.
Therefore, if a desire exists to set the excitation and emission value for the read at the determined spectral peaks, a bandwidth of 12 nm can then be incorporated to yield the highest S:N ratio, the lowest LOD and more than sufficient sensitivity for produced water effluent analysis.
Sample 201 Monochromator Setting Optimization: Off Peak Assessment
While setting excitation and emission values at the spectral peak may be a desire by the end user, it may not always yield the highest S:N ratios and most sensitive read parameters. This is particularly true when the fluorescent molecule has a narrow Stokes shift, leading to a larger overlap between excitation and emission spectra, as seen with sample 201. Therefore, it is also important to test settings that minimize unwanted crosstalk, but also allow for the most optimal inclusion of wavelength ranges that will create the most sensitive read possible for the fluorescent probe.
In the case of sample 201, parameters were tested such that the highest included excitation wavelength and lowest included emission wavelength were fixed at the peak determined values of 494 and 517 nm, respectively, in accordance with the 23 nm Stokes shift. The set excitation and emission values were then moved appropriately to the left and right off of the peaks to allow for incorporation of wider bandwidth ranges without issues with crosstalk (Figure 4).
Figure 4. Sample 201 Off Peak Variable Bandwidth Wavelength Coverage. Constant 23 nm gap maintained between included excitation and emission wavelengths. Wavelength coverage shown when excitation and emission bandwidths set to 3, 6, 9, 12, 15, 20, 30, 40, and 50 nm bandwidths.
S:N ratios were once again calculated as previously described. Upon examination of the results in Figure 5, it is obvious that the same pattern is seen as when performing the on peak optimization. Allowing an increased amount of light to excite the sample, along with capturing a larger range of the emission signal increases S:N ratios. A maximum is reached using a 20 nm bandwidth, wherein use of larger bandwidths begins to suffer increased background, lowering S:N ratios. However, what is also clear is that by minimizing the capture of unwanted crosstalk, background levels are further diminished, compared to the use of excitation and emission settings on the spectral peaks. S:N ratios can reach as much as ten-fold higher than what could be achieved during the previous test.
Figure 5. Off Peak Sample 201 Calculated Signal:Noise Ratio Values. Excitation and emission values set appropriately to maintain a 23 nm gap between included wavelengths and incorporate bandwidths ranging from 3-50 nm.
Higher S:N ratios also yield LOD values at the ppt level that are up to 10X lower than those attained using on peak monochromator settings (Table 2).
Table 2. Sample 201 Off Peak Extrapolated Limit of Detection Values.
The results of the complete set of reads performed using sample 201 confirm that LOD requirements can be met and easily exceeded by about a thousand fold using appropriately optimized monochromator settings, even when incorporating a fluorescent probe with a narrow Stokes shift.
Sample 204 Fluorescent Probe Spectral Profile
A second sample was also assessed to determine the optimum monochromator settings. A spectral scan was again carried out using 1 nm increments to capture the shape and intensity of both spectra.
Figure 6. Sample 204 Excitation and Emission Spectra.
When plotting the normalized signal from the spectral scans, it can be seen that as opposed to sample 201 whose spectral curves contained steep shoulders and a narrow Stokes shift, the curves for sample 204 contain much broader shoulders and a wide Stokes shift of 67 nm.
Sample 204 Monochromator Setting Optimization: On Peak Assessment
Excitation and emission values in a read step were set at the previously determined spectral peaks for the probe, 616 nm and 683 nm, respectively. Multiple bandwidth values were once again evaluated from the minimum wavelength range of 3 nm to the maximum of 50 nm (Figure 7).
Figure 7. Sample 204 On Peak Variable Bandwidth Wavelength Coverage. Included wavelengths shown when 616 and 683 nm excitation and emission values chosen respectively, and bandwidths set to 3, 6, 9, 12, 15, 20, 30, 40, or 50 nm.
Sample 204 was diluted in an equivalent manner as the previous sample, and read in the same 96-well black plate type using 10 measurements per data point and a delay after plate movement of 100 milliseconds.
Figure 8. On Peak Sample 204 Calculated Signal:Noise Ratio Values. Excitation and emission set to determined peak values of 616 and 683 nm, respectively.
Upon calculation and plotting of the average S:N values in relation to the known [ppb] (Figure 8), it is evident that expanding bandwidth increases S:N ratios until a plateau is reached with the incorporation of a 30, 40, or 50 nm range. This can be attributed to the wide Stokes shift of the fluorescent probe. This allows areas of the excitation and emission curves yielding the highest percent fluorescence to be included in the read step without the concern of increasing background signal due to capture of high levels of unwanted crosstalk, as seen with sample 201.
The extrapolated LOD values in Table 3 confirm this point, as the reads demonstrating the greatest sensitivity incorporate 30-50 nm bandwidths.
Table 3. Sample 204 On Peak Limit of Detection Values. Excitation and emission values set at 616 nm and 683 nm, respectively.
Due to the wide Stokes shift and low level of crosstalk, it is also not necessary to move excitation and emission settings off of the determined peaks for the fluorescent probe. The results above again demonstrate that monochromator settings can be optimized, this time using a fluorescent probe with a wide Stokes shift, that exceed established LOD requirements.
The adoption of fluorescent probes to detect trace crude oil levels and other potential pollutants in effluent water streams can greatly simplify analysis procedures, and also deliver levels of sensitivity not attainable with previous methods. With the addition of a monochromator-based microplate reader, which incorporates variable bandwidth monochromators, the process can be enhanced to an even greater degree. Monochromator-based detection allows for levels of multiple pollutants to be determined simultaneously, without requiring changes to the reader setup. The variable bandwidth feature then enables numerous levels of optimization to be performed to find the ideal settings for each fluorescent probe to ensure the most robust, sensitive read parameters are achieved.