We use cookies to provide visitors of our website with the best possible experience. To learn more how we use cookies or how to block cookies, please visit our cookie policy.

Resources - Application Notes

α-SMA Expression in Hepatic Stellate Cells


Related Products: BioSpa 8, Cytation 5

December 21, 2018

Cisbio logo

A comprehensive exploration based on phenotypic and protein observations


α-smooth muscle actin (α-SMA} is a protein that is encoded by the gene ACTA2 in humans, and is a key component of the cellular contractile system. α-SMA expression is considered a reliable marker of hepatic stellate cell (HSC} activation and a key biomarker for liver fibrosis. In the liver, HSCs play a central role in the development of liver fibrosis, transdifferentiating from "quiescent" HSC to a myofibroblastic phenotype in response to Transforming Growth Factor beta (TGF-β), inflammatory processes and ROS.

In this application note, the Biotek Cytation™ 5 Cell Imaging Multi-Mode Reader and BioSpa were used to follow the expression of α-SMA in Human LX-2* cells in response to TGF-β stimulation. Phenotypic changes were captured using the instruments' immunofluorescent and live imaging capabilities. Protein expression of α-SMA was confirmed using the HTRF α-SMA kit. These visual and relative observations demonstrate the pivotal role played by α-SMA during myofibroblast differentiation.