Resources - Application Bulletins
BioTek Resources for Agilent pH-Xtra Glycolysis AssayDownload
Related Products: Cytation 1, Cytation 5, Synergy H1, Synergy Neo2
June 27, 2018
The Agilent pH-Xtra™ Glycolysis Assay allows direct, real-time, kinetic analysis of extracellular acidification rates (ECA/ECAR). BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation 1, Cytation 5, or Synergy H1, or Synergy Neo2 instruments equipped with TRF capability.
Figure 1. Example data of a glucose oxidase titration performed on the Synergy H1 illustrates the detection principle of the Agilent pH probe: higher lifetime signals (left) equate to lower sample pH values (middle). pH values can then be converted to H+ and ECAR using Gen5 software (right).
Figure 2. A summary of the AgilentpHX.prt procedure, plate layout, and data reduction steps as shown from within Gen5 v3.04
Figure 3. Top level implementation steps for the Agilent pH-Xtra Glycolysis Assay with the BioTek Cytation 1, Cytation 5, Synergy H1, or Synergy Neo2 instruments. The toolkit is designed for models equipped with Time Resolved Fluorescence (TRF) capability.