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Resources - Application Bulletins

BioTek Resources for Agilent pH-Xtra™ Glycolysis Assay

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Related Products: Cytation 1, Cytation 5, Synergy H1, Synergy Neo2

June 27, 2018

Red arrowRelated Sample File: AgilentXpH.prt, pH-Xtra Glycolysis.xml

Introduction

The Agilent pH-Xtra™ Glycolysis Assay allows direct, real-time, kinetic analysis of extracellular acidification rates (ECA/ECAR). BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation 5, or Synergy™ H1, or Synergy Neo2  instruments equipped with TRF capability.
 

 
8040588 cube
Filter Cubes:
P/N 8040588 FLTR BLK PH-XTRA GLYCOLYSIS ASBY (Cytations, Synergy H1)
P/N 1035124 CUBE 124 PH-XTRA GLYCOLYSIS ASBY (Synergy Neo2)
Gen5
Gen5 Data Analysis Protocol: AgilentpHX.prt
Note: The User Tutorial and Data Analysis Protocol are compatible with Gen5 v3.04 and higher software. Existing Gen5 users can upgrade their software for free at: https://btresource.force.com/CRC/s/


Example data of a glucose oxidase

Figure 1. Example data of a glucose oxidase titration performed on the Synergy H1 illustrates the detection principle of the Agilent pH probe: higher lifetime signals (left) equate to lower sample pH values (middle). pH values can then be converted to H+ and ECAR using Gen5 software (right).

A summary of the AgilentpHX.prt procedure, plate

Figure 2. A summary of the AgilentpHX.prt procedure, plate layout, and data reduction steps as shown from within Gen5 v3.04
software.

Top level implementation steps of the Agilent pH-Xtra Glycolysis Assay toolkit for

Figure 3. Top level implementation steps for the Agilent pH-Xtra Glycolysis Assay with the BioTek Cytation 1, Cytation 5, Synergy H1, or Synergy Neo2 instruments. The toolkit is designed for models equipped with Time Resolved Fluorescence (TRF) capability.

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