BioTek Resources for Agilent MitoXpress® Xtra Oxygen Consumption Assay

Introduction

The Agilent MitoXpress® Xtra Oxygen Consumption Assay allows real-time measurement of oxygen consumption of whole cells or isolated mitochondria. BioTek offers a dedicated filter cube assembly, pre-programmed Gen5 protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation 1, Cytation 5, Synergy H1, or Synergy Neo2 instruments equipped with TRF capability.
 

Agilent MitoXpress Xtra Oxygen Consumption Assay User Tutorial

Filter Cubes:
P/N 8040587 or P/N 8040594 (Cytation, Synergy H1)
P/N 1035123 CUBE 123 MITOXPRESS XTRA ASBY (Synergy Neo2)

Gen5 Data Analysis Protocol: AgilentXO2.prt
Note: The User Tutorial and Data Analysis Protocols are compatible with Gen5 v3.04 and higher software. Existing Gen5 users can upgrade their v3.0x software for free at: https://btresource.force.com/CRC/s/

Figure 1 - Example Data (Cytation 5): Lifetime detection of extracellular oxygen consumption in HepG2 cells (left). Slope is calculated from the linear portion of the curves to calculate relative oxygen consumption rates (OCR) of various cell treatments (right).

Figure 2. A summary of the AgilentpHX.prt procedure, plate layout, and data reduction steps as shown from within Gen5 v3.04 software.

Figure 3. Top level implementation steps for the Agilent MitoXpress Xtra – Oxygen Consumption Assay with the BioTek Cytation 1, Cytation 5, Synergy H1, or Synergy Neo2 instruments. The toolkit is designed for models equipped with Time Resolved Fluorescence (TRF) capability.