Resources - Application Bulletins

BioTek Resources for Agilent MitoXpress® Intra Intracellular Oxygen Assay


Related Products: Cytation 1, Cytation 5, Synergy H1, Synergy Neo2

January 29, 2019

Red arrowRelated Sample Files: AgilentIntraO2 and MitoXpress


The Agilent MitoXpress® Intra Intracellular Oxygen Assay allows real-time measurement of intracellular oxygen levels of whole cells in 2D or 3D cell cultures. BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation™ 5, Synergy™ H1, or Synergy™ Neo2 instruments equipped with TRF capability.

8040588 cube
Filter Cubes:
P/N 8040587 or 8040594 (Cytation, Synergy H1)
P/N 1035123 CUBE 123 MITOXPRESS ASBY (Synergy Neo2)
Gen5 Data Analysis Protocol: AgilentIntraO2.prt
Note: The User Tutorial and Data Analysis Protocol are compatible with Gen5 v3.04 and higher software. Existing Gen5 users can upgrade their software for free at:

Example data of a glucose oxidase

Figure 1. Example Data (Cytation 5): (A.) Lifetime detection of intracellular oxygenation in HepG2 cells during stepped decreases in oxygen within the read chamber. (B.) Intracellular O2 concentration can be calculated from
a first order exponential fit on the lifetime values and is shown compared to the applied instrument oxygen levels (orange).

A summary of the AgilentpHX.prt procedure, plate

Figure 2. Top level implementation steps for the Agilent MitoXpress Intra Intracellular Oxygen Assay resources for Time-Resolved Fluorescence (TRF) equipped BioTek Cytation™ 1, Cytation™ 5, Synergy™ H1, or Synergy™ Neo 2 instruments.

Top level implementation steps of the Agilent pH-Xtra Glycolysis Assay toolkit for

Figure 3. The AgilentIntraO2.prt procedure, plate layout, and data reduction steps as shown from within Gen5™ software.