Resources - Application Bulletins
BioTek Resources for Agilent MitoXpress® Intra Intracellular Oxygen AssayDownload
Related Products: Cytation 1, Cytation 5, Synergy H1, Synergy Neo2
January 29, 2019
The Agilent MitoXpress® Intra Intracellular Oxygen Assay allows real-time measurement of intracellular oxygen levels of whole cells in 2D or 3D cell cultures. BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation™ 5, Synergy™ H1, or Synergy™ Neo2 instruments equipped with TRF capability.
Note: The User Tutorial and Data Analysis Protocol are compatible with Gen5 v3.04 and higher software. Existing Gen5 users can upgrade their software for free at: https://btresource.force.com/CRC/s/article/Gen5-3-05
Figure 1. Example Data (Cytation 5): (A.) Lifetime detection of intracellular oxygenation in HepG2 cells during stepped decreases in oxygen within the read chamber. (B.) Intracellular O2 concentration can be calculated from
a first order exponential fit on the lifetime values and is shown compared to the applied instrument oxygen levels (orange).
Figure 2. Top level implementation steps for the Agilent MitoXpress Intra Intracellular Oxygen Assay resources for Time-Resolved Fluorescence (TRF) equipped BioTek Cytation™ 1, Cytation™ 5, Synergy™ H1, or Synergy™ Neo 2 instruments.
Figure 3. The AgilentIntraO2.prt procedure, plate layout, and data reduction steps as shown from within Gen5™ software.