Overcoming Pitfalls in Live Cell Imaging
Wednesday, June 1, 2022, 12 PM EDT
Presenters: Jamie Stern, PhD. and Allison Cross, PhD., Field Applications Scientists
The study of complex and dynamic cellular behaviors requires monitoring of cells in real time. Depending on the cellular behavior of interest, these studies may involve monitoring rapid changes occurring over seconds or long-term events over hours or days. For both short duration and longer-term live cell imaging assays, there are many factors that need to be considered for optimal experiment design and execution. In this webinar, we will discuss some of the common pitfalls that scientists encounter when setting up their live cell imaging assays and how to overcome them.
Key Learning Objectives:
- Optimizing sample preparation (from plate choice to seeding density)
- Reagent selection (including media and fluorescent labels)
- Overcoming bleaching and photo-toxicity
- Avoiding unnecessary oversampling
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