Life science instrumentation is used in a vast number of applications across multiple scientific disciplines. Through our Webinar presentations, BioTek seeks to share information and experiences with attendees about timely and important applications. Sign up today and check back for future topics that may be of interest to you.
ELISA: Best Practices and Troubleshooting
Presenter: Paul Held, Ph.D., Applications Laboratory Manager
Date: Wednesday, September 20, 2017 Time: 12 PM EDT
Enzyme-linked immunosorbent assay (ELISA) is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process. The reaction uses a solid surface, typically a microplate, to immobilize the analyte. Once immobilized, unwanted materials can be removed by washing and the remaining analyte quantitated. The assay technology uses antibodies in conjunction with enzyme-catalyzed amplification to provide high specificity and high sensitivity. Here we will describe the basic process steps of ELISA, best laboratory practices along with troubleshooting tips for common problems. In addition, some of the devices and instrumentation used with this assay technology will be described.
For more information and to register, click here.
3-Dimensional Assays: What Must Be Done for Setting Up and Validating for Downstream Microplate Reader and Imaging Applications
Presenters: Brad Larson, Principal Scientist, BioTek Instruments Inc. and Mark Rothenberg, Ph.D., Manager Scientific Training and Education, Corning Incorporated, Life Sciences
Date: Tuesday, September 26, 2017 Time: 11 AM EDT
Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from our standard 2-dimensional (2D) systems to a more in-vivo-like or 3-dimensional (3D) environment. The advance of new technologies and screening methodologies have allowed scientists to assess more realistic cellular functionality. Today’s webinar will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.
Read more about the topic in the SLAS Discovery Special Issue on 3D Cell Culture, Drug Screening and Optimization (featuring free access to select articles courtesy of Corning) and the SLAS Electronic Laboratory Neighborhood feature article. Register today!
For details and to register, visit the SLAS Webinar webpage.