Variable Bandwidth Monochromators
Many multi-mode microplate readers use monochromator-based optics for fluorescence intensity measurements, allowing great flexibility to use multiple fluorescent dyes and stains. Most of these systems offer a fixed bandwidth, which works well for standard fluorophores but aren’t always optimal for some others, including those with a narrow Stokes’ Shift.
Synergy™ Neo2 Multi-Mode Reader, Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 and Cytation 7 Cell Imaging Multi-Mode Readers have monochromator optics that incorporate user-selectable variable bandwidths for the best assay specificity and assay sensitivity. Synergy Neo2’s monochromators have a range from 3 nm to 50 nm, while the Synergy H1, Cytation 5 and Cytation 7 have a range of 9 nm to 50 nm; both ranges are selectable in 1 nm increments.
Click on the videos below to learn more about the benefits of variable bandwidth monochromators for fluorescence detection.
Learn more about optimizing applications using variable bandwidth in BioTek’s multi-mode microplate readers.
- Utility of Variable Bandwidth Monochromators for Quantification of Fluorescent Probes in Produced Effluent Water
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