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Gen5 Software Features for Imaging & Microscopy

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BioTek Gen5 Software for imaging and microscopy offers automated image capture, processing and analysis for a broad range of samples, from whole organism imaging to high magnification subcellular imaging. Raw data and images are transformed into meaningful results with powerful built-in capture, process and analysis tools. The automated process, known as Augmented Microscopy, enables simple and straightforward imaging and analysis workflows.

Highly visual workflow interface in Gen5
Highly visual workflow interface in Gen5 3.04


Gen5 Software supports applications enabled with BioTek Lionheart Automated Microscopes and Cytation Cell Imaging Multi-Mode Readers. From image capture and quantitative data collection through, processing and analysis to publication-ready images, movies and data, Gen5 provides an easy, seamless workflow in an uncomplicated interface. Key applications are highlighted in these visual abstracts:


Autophagy Analysis Using Object Spot Counting
Autophagy Analysis Using Object Spot Counting

Cell proliferation label-free
Cell Proliferation - Label-free Confluence

Mitochondrial Membrane Potential Analysis Using Object Spot Counting
Calcium Kinetics

3D NK cells
3D Kinetic NK Cell Cytotoxicity Assay

See other Gen5 supported imaging and microscopy applications on our Applications pages.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!



   Automated Image Capture

 Automated Image Capture
 Automated Image Capture

Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.


Exceptional Image Processing Tools

Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications. The independent optical paths ensure uncompromised performance in all modes.  

   Powerful Image Analysis

 Label-free cell counting

Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.


   Publication-ready Images, Graphs and Data


Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.

Automated Image Capture


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