product design

Synergy 2 Multi-Mode Reader

Specifications

General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha
Read methods End point, kinetic, spectral scanning, well-area scanning
Microplate types 6- to 1536-well plates
1- to 384-well (luminescence)
Other labware supported PCR plates, Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 65 °C; +0.2 °C at 37 °C
Shaking Linear
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible

Absorbance

Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 - 999 nm, 1 nm increment
Monochromator bandwidth 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
1536 wells: 42 seconds

Fluorescence Intensity

Light source Tungsten halogen
Xenon flash (option)
Detector PMT
Wavelength selection Filters/ dichroic mirrors
Wavelength range Tungsten lamp: 300 - 700 nm (850 nm option)
Xenon lamp: 200 - 700 nm (850 nm option)
Dynamic range >6 decades
Sensitivity Top: Fluorescein 1 pM (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate)
Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate)
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
1536 wells: 42 seconds

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash)
100 amol ATP (glow)

Fluorescence Polarization

Light source Tungsten halogen
High energy DPR Xenon flash (optional)
Detector PMT
Wavelength selection Filters/dichroics
Wavelength range 400 - 700 nm (320 - 850 nm option)
Sensitivity 3 mP at 1 nM fluorescein

Time-Resolved Fluorescence

Light source Xenon flash
Detector PMT
Wavelength selection Filters/dichroics
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Europium 60 fM (12 amol/well 96-well plate; 6 amol/well 384-well plate)

Alpha Detection

Light source Tungsten halogen
Detector PMT
Wavelength selection Filters
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
Read speed 2 minute (96-well plate)

Reagent Dispensers

Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates
Dead volume 1.1 mL, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 nm increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Power 250 Watts max.
Dimensions 17"W x 17.5"D x 14.5"H
(43.5 x 44.5 x 37.3 cm)
Weight 60 lbs (27 kg)

Regulatory

Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

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