product design

Synergy HTX Multi-Mode Reader

Specifications

General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Time-resolved fluorescence (secondary mode)
Alpha
Read methods End point, kinetic, spectral scanning and well area scanning
Microplate types 6- to 384-well plates
Other labware supported PCR plates, Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 50 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible

Absorbance

Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 - 999 nm, 1 nm increments
Monochromator bandwidth 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 14 seconds
384 wells: 26 seconds

Fluorescence Intensity

Light source Tungsten halogen
Xenon flash (option)
Detector PMT
Wavelength selection Filters
Wavelength range 300 - 700 nm (200 - 850 nm option)
Dynamic range >6 decades
Sensitivity Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate)
Reading speed (kinetic) 96 wells: 31 seconds
384 wells: 80 seconds

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash) - Lum and Abs / Lum configurations
30 amol ATP (flash) - Multi-mode configurations

Time-Resolved Fluorescence

Light source Xenon flash
Detector PMT
Wavelength selection Monochromator

Alpha Detection

Light source Tungsten halogen
Detector PMT
Wavelength selection Filters
Sensitivity 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
Read speed 2 minute (96-well plate)

Reagent Dispensers

Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates
Dead volume 1.1 mL with back flush
Dispense volume 5-1000 µL in 1 nm increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Connectivity 1 USB, 1 RS232 for external PC control
Power 100-240 VAC, 50/60 Hz
Dimensions 16"W x 15"D x 10"H
(40.6 x 38 x 25.4 cm)
Weight 40 lbs (18 kg)

Regulatory

Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

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